Figure 4

Oxaliplatin induces nucleolar segregation and axonopathy. (a, b) Light microscopy revealed that DRG neurons from mice treated with oxaliplatin 3.5 mg/kg/iv twice weekly for four weeks (b; n = 3) had segregated nucleoli (arrow heads) that were eccentric (black arrow) compared to DRGs from naïve mice (a; n = 3). (c, d) Electron microscopy also showed segregated nucleoli in DRG neurons from oxaliplatin-treated mice (d; n = 3) but not naïve mice (c; n = 3). Light microscope examination demonstrated that myelinated fibers in the sciatic nerve of mice treated with oxaliplatin 3.5 mg/kg/iv twice weekly for four weeks (f; n = 3) had evidence of changes indicative of axonopathy (white arrows) that were not found in sciatic nerve from naïve mice (e; n = 3).