Figure 1

TRPV1 association with AKAP150 is calcium sensitive. (A) Co-immunoprecipitation and Western blot analysis of TRPV1 and AKAP150 following A23187 (1 μM, 10 min) in trigeminal neurons. (B) Quantified densitometry of data represented in (A), n = 4. (C) Plasma membrane expression of TRPV1 and AKAP150 does not change following A23187 exposure. (D) Quantified densitometry of data represented in (C), n = 3. (E) Co-immunoprecipitation of TRPV1 and AKAP150 from cultured TG neurons pretreated with A23187 and/or W-7 (calmodulin antagonist (100 μM; 30 min). (F) Quantified densitometry of data represented in (E), n = 5. Significance to vehicle treatment determined by two-tailed, paired t-test, **p < 0.01.