Intracellular calcium effects TRPV1 and AKAP150 association and TRPV1 activity. (A) Co-immunoprecipitation and Western blot analysis from CHO cells following BAPTA-AM (50 μm, 10 min) or A23187 (1 μm, 10 min). (B) Quantified densitometry of co-immunoprecipitation normalized to total TRPV1 expression. (C) Representative calcium-imaging traces of capsaicin (CAP)-stimulated (50 nm, 30 sec) intracellular Ca2+ accumulation from CHO cells expressing TRPV1 and AKAP150. Cells pretreated with vehicle (H20, veh), BAPTA-AM (50 μm, 10 min) or A23187 (1 μm,10 min) followed by 8-Br-cAMP (10 μm, 30 sec) administration. (D) Data represented in C are quantified as ΔF340/F380, mean ± SEM shown, n shown for each treatment paradigm. Significance determined by one-way ANOVA with Bonferroni correction, NS = no significance, *** p < 0.001.