Figure 6

Calmodulin co-expression decreases TRPV1 and AKAP150 TIRF/FRET signal. (A) Images of CHO cells expressing CFP-tagged TRPV1 and YFP-tagged AKAP150 and either wild-type calmodulin (wtCaM) or dominant-negative calmodulin (DNCaM) under TIFR illumination, using 442 or 514 nm laser lines. Images of the CFP (in rainbow pseudocolor) and YFP (in yellow pseudocolor) emissions are shown before or after YFP photobleach, as labelled. (B) TIRF/FRET values from images acquired in A are quantified and illustrated as percent increase in CFP emission cells expressing the indicated calmodulin species. Vector = pcDNA3, mean ± SEM shown. Significance determined by Student's T-test, n = 9-12 cells/transfection paradigm, *p < 0.05.