Figure 2

Temperature preference and heat-evoked nociceptive behavior of TRPV3 knockout mice on the 129S6 background. (A) Thermal preference behavior of wild-type (black solid line, n = 12) and TRPV3 knockout (KO, red dashed line, n = 10) mice on a floor temperature gradient of 0.8 to 48.8°C. Percent of time (mean ± SEM) spent within the indicated 2°C bins was monitored during the indicated time periods. Right panel shows percent of time (mean ± SEM) spent by wild-type (filled bar) and knockout (open bar) mice between 19°C and 29°C during the final 30 min. (B) Preference index of individual mice for temperatures > 29°C in the thermal gradient. Wild-type C57BL6 mice were those presented in Figure 1A (grp 1, n = 11) or a separate group (grp 2, n = 7) assayed at the same time as the 129S6 mice from Figure 2A (129S6 wild-type n = 12, 129S6 TRPV3 KO n = 10). (C) Thermal preference of wild-type (filled black squares, n = 10-12) and TRPV3 KO (open red circles, n = 10-12) mice in two-temperature selection tasks. Percent of time spent (mean ± SEM) at the indicated temperature was monitored at 5 min intervals. (D) Heat-evoked acute nociceptive behavior of wild-type (filled bars, n = 12) and TRPV3 KO (open bars, n = 10) mice. Latency to response was measured in the tail immersion (left), hot plate (middle), and radiant paw heating (right) assays. Data represent mean ± SEM. (* p < 0.05, wild-type vs. knockout, unpaired t-test).