Figure 3
From: TRPV3 and TRPV4 ion channels are not major contributors to mouse heat sensation
Temperature preference and heat-evoked nociceptive behavior of TRPV3/TRPV4 double knockout mice on the C57BL6 background. (A) Thermal preference behavior of freely moving wild-type (black solid line, n = 12) and TRPV3/TRPV4 double knockout (V3V4 KO, red dashed line, n = 10) mice on a floor temperature gradient of 0.8°C to 48.8°C over 120 minutes. Percent of time spent (mean ± SEM) within the indicated 2°C bins was monitored during the indicated time periods. (B) Heat-evoked acute nociceptive behavior of wild-type (filled bars) and TRPV3/TRPV4 double knockout (open bars) mice. Latency to response was measured in the tail immersion (left, n = 12-13) and hot plate (middle, n = 12) assays at the indicated temperatures. Latency to response in the radiant paw heating assay (n = 19-20) was measured during stimulation at a fixed lamp intensity. Data represent mean ± SEM. (*p < 0.05 wild-type vs. knockout, unpaired t-test).