Figure 2
Mechanical allodynia (von Frey filaments). The 50% paw withdrawal threshold (PWT) to a static mechanical stimulus was assessed using von Frey filaments and the up-and-down method. Rats received intra-tibial inoculations of two kinds of Walker 256 cell suspension (1 × 105 cells/5 μl Hank's solution) (group V1 and group A1), heat-killed carcinoma cells in 5 μl Hank's solution (group K1), or 5 μl Hank's solution only (group N1). PWT was measured pre-surgery (Baseline, BL) and on days 3, 6, 9, 12, 15, and 18 after cell inoculation (Figure 2). All data are expressed as means ± SEM. **P < 0.01 vs. group N1. ## P < 0.01 vs. group K1.