Table 1 Sequences of TaqMan probe, forward primer and reverse primer of nNOS, iNOS, IL-6, TLR4 and P2X4R for real time quantitative RT-PCR
| Â | probe and primers | Sequence |
|---|---|---|
nNOS | probe | 5'-FAM-TTGGCCCAGGCACCGGCA-TAMRA-3' |
| Â | forward primer | 5'-TTCCACCTGCCTCGAAACC-3' |
| Â | reverse primer | 5'-GCACGTCCTGTACATATTTCTTTGG-3' |
iNOS | probe | 5'-FAM-CCCCCAGCGGAGTGACGGC-TAMRA-3' |
| Â | forward primer | 5'-ACATCAGGTCGGCCATCACT-3' |
| Â | reverse primer | 5'-CGTACCGGATGAGCTGTGAAT-3' |
IL-6 | probe | 5'-FAM-TCACAGAGGATACCACTCCCAACAGACCTG-TAMRA-3' |
| Â | forward primer | 5'-GGGACTGATGCTGGTGACAA-3' |
| Â | reverse primer | 5'-TGCCATTGCACAACTCTTTTCT-3' |
TLR4 | probe | 5'-FAM-CACGTCCATCGGTTGATCTTGGGAGAA-TAMRA-3' |
| Â | forward primer | 5'-AAACTTGCCTTCAAAACCTGGC-3' |
| Â | reverse primer | 5'-ACCTGAACTCATCAATGGTCACATC-3' |
P2X4R | probe | 5'-FAM-CAATGAGCAACGCACACTCACCAAGG-TAMRA-3' |
| Â | forward primer | 5'-ACAACGTGTCTCCTGGCTACAAT-3' |
| Â | reverse primer | 5'-GTCAAACTTGCCAGCCTTTCC-3' |