Table 1 Antibodies, dilutions and sources
From: Accumulation of Kv7.2 channels in putative ectopic transduction zones of mice nerve-end neuromas
Antibody | Immunogen | Manufacturer, species antibody was raised in, mono- vs. Polyclonal | Dilution used |
|---|---|---|---|
Pan Nav | 1500-1518 aa of the α-subunit of Voltage Gated Sodium Channels | Chemicon, Rabbit, polyclonal | 1:800 |
Myelin P2 | The epitope for sc-49303 maps within amino acids 50-100 in the internal region of Myelin P2 of human origin (accession #P02689) | Santa Cruz, Goat, Polyclonal | 1:100 |
CASPR | Fusion protein 1308-1381 (cytoplasmic domain) of rat Caspr (accession number #P97846) | NeuroMab (UC Davis, Davis CA), mouse monoclonal, clone K65/35 | 1:500 |
Pan NF | Fusion protein 1066-1174 (intracellular domain common to NF -155 and NF-186) of rat NF-155 (accession number AAL27854) | NeuroMab (UC Davis, Davis CA), mouse monoclonal, clone L11A/41 | 1:500 |
Kv7.2 | aa 578-593 at the C-terminal domain | Alomone, rabbit, polyclonal | 1:200 |
Kv7.2 | aa 578-593 at the C-terminal domain | Chemicon, rabbit, polyclonal | 1:400 |
Kv7.2 | aa 578-593 at the C-terminal domain | Sigma, rabbit, polyclonal | 1:200 |
Kv7.2 | C-18; The epitope for sc-7792 maps within the last 50 amino acids at the C-terminus of KCNQ2 of human origin (accesion #O43526) | Santa Cruz, goat, polyclonal | 1:800 |
Kv7.2 | N-19; The epitope for sc-7793 maps within the first 50 amino acids at the N-terminus of KCNQ2 of human origin (accession #O43526) | Santa Cruz, goat, polyclonal | 1:1000 |