Figure 6

EGFP expression in subpopulations of primary sensory neurons following VSV-G pseudotyped EF1α-EGFP vector injection into DRG. 4 weeks after VSV-G LV2-EF1α-EGFP vector injection ipsilateral DRG sections were immunostained with EGFP antibody (green, A, D, G, J, M, P) to detect EGFP expressing cells. Neurons were identified by staining for β3-tubulin (β3-Tub, red, B). Neurofilament 200 (NF200, red, E) labeled myelinated neurons, isolectin IB4 (IB4, red, H) labeled non-peptidergic small neurons, α-calcitonin gene-related peptide (CGRP, red, K) labeled peptidergic small neurons, and transient receptor potential vanilloid 1 (TRPV1, red, N) labeled nociceptive neurons. Satellite glia were labeled with glutamine synthetase (GS, red, Q). Arrowheads are examples of EGFP-immunoreactive cells double labeled for a given neuronal marker, evident in the overlays (yellow, C, F, I, L, O). In P, Q, and R, the arrowheads denote EGFP-immunoreactive neurons negative for GS, while the arrows indicates rings of satellite glial cells. Scale bar: 100 μm.