Expression pattern of sEH in primary afferent neurons. (A) Representative western blot analysis of sEH-expression during zymosan induced inflammation in paw and DRG-tissue 0 - 48h after zymosan injection. (B) Western blot detection of sEH expression in tissues from dorsal root ganglia and paw oedema tissue of wild type and sEH-deficient mice 48h after zymosan injection. Arrowhead indicates a signal appearing only in wild- type tissue at approximately 58 kDa. (C) Expression pattern of sEH in dorsal root ganglia neurons. Immunofluorescence staining of L4 or L5 dorsal root ganglia cryosections during zymosan induced inflammation (0h, 1h, 6h and 48h zymosan) was performed with antibodies against sEH (green) and NeuN (red). At time point 48h post zymosan injection wildtype and sEH-deficient DRG-tissue were compared. (D) Selective sEH expression in NF200 positive neurons. Combination of conventional immunofluorescence staining for sEH as in (C) with the MELC technology using 7AAD and FITC-labeled primary antibodies against NeuN, glial fibrillary acidic protein (GFAP), isolectin B4 (IB4) and neurofilament 200 (NF200).