Contribution of sEH to EET release from sensory neurons and DRG tissue levels during inflammation. (A) Basal EET levels extracted from tissues of DRGs from L4 and L5 segments from wild type and sEH deficient mice were determined by LC-MS/MS analysis. (B) EET-tissue levels in DRGs 48h after intraplantar zymosan injection. Data shown represent the mean ± SEM from 6 animals. (C) EET and DHET (D) levels in lysates of sensory neurons. Neuron enriched cultures from DRGs of wild- type and sEH-deficient mice were stimulated with 0.5 μM arachidonic acid and incubated for 2 h until extraction of EETs from cells. Data shown represent the mean ± SEM from 5 culture dishes. (E) EET and DHET (F) release from sensory neurons.. EETs were extracted from culture media of cells used in panel (C). Data shown represent the mean ± SEM from 5 culture dishes. Student's t test: *, p ≤ 0.05; **, p ≤ 0.01.