Effects of EETs and sEH deletion on mechanical pain thresholds. (A) 8,9-EET induces mechanical hyperalgesia. 20 μl of 10 μM 8,9-EET was injected intraplantar and mechanical thresholds were determined by the Dynamic Plantar Test. Control animals received vehicle solution containing the corresponding volumes of ethanol (3.2% v/v) and were tested in parallel. Data shown represent the mean ± SEM from 8 animals per group. Two way Anova with Bonferroni post test: *, p ≤ 0.05; **, p ≤ 0.01. (B) Same experiments than in (A) but other EET regioisomers were used. (C) Thermal thresholds after 8,9-EET injection. 20 μl of 10 μM 8,9-EET or vehicle was injected and thermal thresholds were determined by the Hargreaves test. Data shown represent the mean ± SEM from 4 animals. (D) Effect of sEH deletion on mechanical hyperalgesia after zymosan injection. Mechanical thresholds of both hind paws were tested by the dynamic plantar test after unilateral intraplantar zymosan injection and compared between sEH-/- mice and wild type C57BL/6 controls. Data shown represent the mean ± SEM from 8-9 animals per group. Two way ANOVA with Bonferroni post test: **, p ≤ 0.01.