LC3 expression in the spinal cord following spinal nerve ligation. (A) LC3 expression was analyzed by Western blot in the hemi-cord contralateral (C) and ipsilateral (I) to the side of ligation, 7 days after surgery. SNL animals showed higher LC3-I expression than sham animals (A). A clear trend towards an increased LC3-I expression in the ipsilateral (I) compared to the contralateral (C) side, together with the appearance of LC3-II, was observed after ligation (A) and confirmed by densitometric analysis (B and C). The slight increase in LC3-I levels and the apparent formation of LC3-II well correlated with α2δ-1 upregulation (A and D). Signals from each band were normalized towards the corresponding GADPH signal. One representative Western blot is shown. Sham: n = 5, SNL: n = 6.