Statistical analysis. Quantitative analysis of Western blots by densitometry confirmed a trend towards an increase in LC3-I (A) and Beclin 1 (C) expression in the ipsilateral (I) compared to the controlateral (C) side of SNL animals 7 days after surgery. In the same animals, this was accompanied by a marked increase in LC3-II levels (B) and associated to strong α2δ-1 upregulation (D). For LC3-I, LC3-II, Beclin 1 and α2δ-1: n = 5. Signals from each band were normalized towards the corresponding GAPDH signal. Values were expressed as mean ± SEM, * p < 0.05.