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Figure 3 | Molecular Pain

Figure 3

From: Specific involvement of atypical PKCζ/PKMζ in spinal persistent nociceptive processing following peripheral inflammation in rat

Figure 3

Expression of PKCζ/PKMζ and phospho-PKCζ/PKMζ. (a) RT-PCR showing expression of PKMζ (481 bp) and PKCζ (361 bp) mRNA isoforms in spinal cord (spinal c.), dorsal roots ganglia (DRG) and in both cortex and hippocampus (Hippo.) in naïve rats. (b) Western immunoblot showing expression of PKCζ in hippocampus (Hippo.), DRG and lumbar spinal dorsal horn (Spinal c.) in naïve rats. ß-III tubulin served as loading control. (c) p-PKCζ/p-PKMζ is co-expressed with IB4 in DRG cells of naïve rats. Scale bars = 50 μm. (d, e) p-PKCζ/p-PKMζ is not co-expressed with (d) IB4 or (e) CGRP, used as specific markers for non-peptidergic and peptidergic C-fibers, respectively, in spinal cord sections from formalin rats. Scale bars = 100 μm.

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Molecular Pain

ISSN: 1744-8069

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