Regional distribution of the mRNAs from the rat exon 11-associated variants A. Four sets of total RNAs were extracted from brain regions dissected from four separate groups of rats. Each group contained 1 or 2 rats depending upon the size of the regions. RT-PCRs were performed using primers designed for amplifying rMOR-1G1, rMOR-1G2, rMOR-1H1, rMOR-1H2, rMOR-1i1, rMOR-1i2, rMOR-1i3 and rMOR-1 as described in the Methods. G3PDH was used as RNA loading control. The PCR products were separated on 1% agarose gel, stained with ethidium bromide and photographed using FluorChem 8000 Image System. Only one of four sets data was shown, while the data from other three sets were shown in Additional files 1, 2 &3. B. Quantification of the PCR products from the four sets of RNAs. The band intensities from the agarose gel were quantified with AlphaEase FC software of the Image System and normalized with the band intensities of G3PDH. The data were graphed using GraphPad Prism 4.0 and analyzed with Two-way ANOVA. The results are shown in Table 1.