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Table 2 Saturation studies with [3H] DAMGO

From: Identification and characterization of seven new exon 11-associated splice variants of the rat mu opioid receptor gene, OPRM1

Clone K D (nM) B max (pmol/mg protein)
rMOR-1 0.39 ± 0.03 0.47 ± 0.07
rMOR-1H1 0.51 ± 0.05 0.40 ± 0.01
rMOR-1H2 0.37 ± 0.02 0.30 ± 0.05
rMOR-1i1 0.36 ± 0.05 0.24 ± 0.02
rMOR-1i2 0.36 ± 0.04 0.26 ± 0.01
rMOR-1i3 0.63 ± 0.06 0.24 ± 0.01
  1. [3H] DAMGO binding was performed in membranes of CHO cells stably expressing the indicated variant constructs. The binding parameters were determined by nonlinear regression analysis. Results are the mean ± S.E.M. of at least three independent determinations. P values determined by one-way ANOVA were 0.0013 for K D and 0.0311 for B max . Tukey post hoc analysis determined that rMOR-1i3 was different from rMOR-1, rMOR-1i1, rMOR-1i2 and rMOR-1H2 (p < 0.001), and that there was no significant difference among the variants in B max .