Clone |
K
D
(nM) |
B
max
(pmol/mg protein) |
|---|
rMOR-1 | 0.39 ± 0.03 | 0.47 ± 0.07 |
rMOR-1H1 | 0.51 ± 0.05 | 0.40 ± 0.01 |
rMOR-1H2 | 0.37 ± 0.02 | 0.30 ± 0.05 |
rMOR-1i1 | 0.36 ± 0.05 | 0.24 ± 0.02 |
rMOR-1i2 | 0.36 ± 0.04 | 0.26 ± 0.01 |
rMOR-1i3 | 0.63 ± 0.06 | 0.24 ± 0.01 |
- [3H] DAMGO binding was performed in membranes of CHO cells stably expressing the indicated variant constructs. The binding parameters were determined by nonlinear regression analysis. Results are the mean ± S.E.M. of at least three independent determinations. P values determined by one-way ANOVA were 0.0013 for K
D
and 0.0311 for B
max
. Tukey post hoc analysis determined that rMOR-1i3 was different from rMOR-1, rMOR-1i1, rMOR-1i2 and rMOR-1H2 (p < 0.001), and that there was no significant difference among the variants in B
max
.
