Resolution of lameness and hyperalgesia after treatment. Photograph of a clinically lame animal and close-up of the hindfeet (a). The animal was given a lameness score of 10 (non-weight bearing). Inflammation can be seen in the right foot and tracking up the lower limb. The magnitude of hyperalgesia (Emax%) on the lame limb on day 0 prior to treatment, and up to 7 days post-treatment is represented as the percentage change from 3 non-affected limbs according to the formula: ((mean response threshold of non-affected limbs - response threshold of lame limb)/mean response threshold of non-affected limbs) × 100. In control animals, the mean percentage change in response threshold measured on one limb relative to the three other limbs was calculated (b). Mean lameness score for animals affected by unilateral lameness on day 0 prior to treatment for lameness, and up to 7 days post-treatment (c). Data are presented as mean values; s.e.d. represents the standard error of the difference. a, b, c Means with disparate letters are significantly different (p < 0.05).