Expression of COX-1, COX-2 and Egr-1 proteins in spinal cord. Western blot analyses of COX-1 (a), COX-2 (b) and Egr-1 (c) protein in spinal cord. Photomicrographs show expression of COX-1, COX-2 and Egr-1 at 70, 72 and 60 kDa, respectively, as expected in a representative control sample (con) and ipsilateral (i) and contralateral (c) spinal cord from an animal affected by unilateral lameness on day 0. Graphs show densitometric quantification of levels of COX-1 (a), COX-2 (b) and Egr-1 (c) protein in ipsilateral and contralateral spinal cord tissues from animals affected by unilateral lameness on day 0 before treatment (n = 6) and 3 days after treatment (n = 6). Bars represent mean ± SEM protein expression (as %) relative to control levels (100%). Significant increase from control: * p < 0.05; significant decrease from day 0: * p < 0.05.