Figure 2

Potentiation of synaptic transmission in the ACC slices by IL-8. (A) Representative recordings of evoked EPSCs in the ACC slices from saline, CFA-injected, and ACC infusion of repertaxin mice (upper). Plot of input-output curves showed the enhancement of synaptic transmission in the ACC of CFA-injected mice and ACC infusion of repertaxin partially inhibited CFA-induced synaptic transmission. n = 9 slices/4 mice in each group, * P < 0.05, ** P < 0.01 compared with saline-injected mice; ^# P < 0.05 compared with CFA-injected mice. (B) Representative recordings of evoked EPSCs in the control and IL-8 incubated ACC slices (upper). Plot of input-output curves showed enhancement of synaptic transmission by IL-8 perfusion; however, ACC infusion of repertaxin partially inhibited IL-8-induced synaptic transmission. n = 8 slices/4 mice in each group, * P < 0.05, ** P < 0.01 compared with control; ^# P < 0.05 compared with IL-8 incubated slices. (C) Representative traces of PPF with an interval of 50 ms recorded in the saline, CFA-injected, and ACC infusion of repertaxin mice (upper). PPF was notably reduced in CFA-injected mice, and ACC infusion of repertaxin reversed the reduced PPF in the ACC of CFA-injected mice. n = 10 slices/4 mice in each group, * P < 0.05, ** P < 0.01 compared with saline-injected mice. (D) Representative recordings of PPF in the control and IL-8 incubated ACC slices (upper). PPF was significantly reduced by IL-8 incubated slices, and ACC infusion of repertaxin reversed the reduced PPF in the ACC of IL-8 incubated slices. n = 9 slices/4 mice in each group, *P < 0.05, ** P < 0.01 compared with control.