Figure 2
From: Minocycline markedly reduces acute visceral nociception via inhibiting neuronal ERK phosphorylation
Representative photomicrographs and graph showing immunoreactivity (IR) of c-Fos in the spinal cord (T5-L2). (A, B) c-Fos expression in the spinal cord of the saline/minocycline-pretreated and acetic acid-treated animals. Insets are high magnification of the open rectangles. The spinal gray matter was divided into the following four regions: (1) laminae I-II, (2) laminae III-IV, laminae V-VI, (4) laminae VII-IX, (5) laminae X. The scale bar denotes 100 μm. (C) The number of c-Fos positive cells in spinal cord following minocycline pre-injection. The enhancement in the number of c-Fos positive cells produced by acetic acid was significantly decreased in spinal cord by minocycline pre-administration. The mean number of c-Fos positive cells was calculated by averaging the total numbers of each region per each section/region. Values are expressed mean ± SEM. +, p < 0.01 versus normal group; *, p < 0.01 and **, p < 0.05 versus control group (ANOVA test with a Fisher's post hoc test)