Figure 4
From: Minocycline markedly reduces acute visceral nociception via inhibiting neuronal ERK phosphorylation
Representative photomicrographs showing immunoreactivity (IR) of phospho-ERK (p-ERK) in the spinal dorsal horn (T12-L5). (A-I) p-ERK and NeuN immunoreactivity in the spinal dorsal horn of the saline-pretreated and acetic acid-treated mice. The p-ERK immunofluorescence was found exclusively in spinal neuron (C; merged image), but not in microglia (D, p-ERK; E, CD11/b; F, merged image) or astrocytes (G, p-ERK; H, GFAP; I, merged image). (J, K) Microglia (J, CD11/b) and astrocytes (K, GFAP) in normal mice. The scale bar denotes 50 μm