Figure 3

Effects of warming on the apparent affinity of TRPA1 to MO and time course for activation and desensitization. A, superimposed whole-cell current traces at -80 mV recorded from HEK293 cells stably expressing rat TRPA1 at 23°C (black) or 39°C (red) in response to 0.1, 0.3 and 1 mM MO. Superimposed traces were obtained from independent cells. Scale bars, 30 s, 0.1 (left), 0.3 (middle) and 0.5 nA/pF (right). B, concentration-dependent relationship of MO-evoked currents at 23°C (white) and 39°C (black). C, 20-80% rise time (left) and decay time constant (τ) (right) of currents evoked by 0.3 or 1 mM MO at 23°C (white) and 39°C (black). P < 0.05 in two-way ANOVA; *P < 0.05 in Bonferroni post-hoc test; n = 4-6.