Figure 7
From: Warmth suppresses and desensitizes damage-sensing ion channel TRPA1
Effects of warming on MO-induced ionic currents and intracellular Ca2+ level in rat trigeminal ganglia (TG) neurons. A (bottom), representative traces evoked by warming (left), MO (300 μ M) at 23°C (middle) or at 39°C (right) in cultured TG neurons; (upper), temperature during the equivalent periods. Vm = -70 mV. Scale bar, 0.5 nA, 1 min. B, data derived from experiments in panel A. Dotted lines represent the threshold determining responsiveness of each group. C, box-and-whisker plot comparing MO-evoked current densities at 23°C and 39°C. Only responders in panel B were quantified. Error bars, 5th to 95th percentile; *P < 0.05 in Mann-Whitney U test; n = 18 at 23°C and n = 11 at 39°C. D, changes in Fura ratio evoked by MO applied at 23°C with (black bars) or without (white bars) pre-emptive heat (39°C, 2 min). P > 0.14 in two-way ANOVA; 77-145 neurons/group.