Figure 4
Expression of P2Y 12 R and GFAP in TG and effect of P2Y 12 R antagonist on GFAP expression in LNC-rats. Photomicrographs of P2Y12R-IR cells (A and D); GFAP-IR cells (B); P2Y12R-IR and GFAP-IR cells (C); NeuN-IR cells (E); P2Y12R-IR and NeuN-IR cells (F) in V3 branch region on day 3 after LNC. Photomicrographs of P2Y12R-IR cells (G); GFAP-IR cells (H) in V3 branch region on day 3 after sham operation. Photomicrographs of GFAP-IR cells following MRS2395 administration for 3 successive days into TG in LNC-rats (I). J and K: Size-frequency histograms illustrating distribution of somata of TG neurons encircled with GFAP-IR cells in V3 branch region on day 3 after operation and following daily successive MRS2395 (18.0 ng/day) or vehicle administration (from day 0 to day 2) into TG in LNC-rats (J) and sham-rats (K) on day 3 after LNC or sham operation. Arrows indicate GFAP-IR cells expressing P2Y12R-IR cells. *: p < 0.05, **: p < 0.01, ***: p < 0.001 (n = 5 in each group, Student's t-test). Scale bars = 50 μm.