Peripheral injection of PAP into Weizhong acupuncture point (“PAPupuncture”) has localized and long-lasting A
R-dependent antinociceptive effects. (A) Noxious thermal and (B) mechanical sensitivity following injection of hPAP (250 mU) into the popliteal fossa. (C) Rotarod tests 24 h before (1 test iteration) and 24 h after (2 test iterations, separated by 40 s) injection of hPAP into the popliteal fossa (250 mU/mouse). No significant differences in rotarod performance between genotypes or treatment. (A-C) Ipsi = ipsilateral hindpaw. Contra = contralateral hindpaw. Contralateral popliteal fossa was not injected. Wild-type (n = 10) and A
R−/− (n = 10) male mice in each cohort. Data are plotted as means ± s.e.m. Paired t-tests were used to compare responses at each time point to baseline (BL). *P < 0.05, **P < 0.005, ***P < 0.0005.