Larger hPAP doses extend the antinociceptive effects of PAPupuncture. (A) Thermal and (B) mechanical sensitivity following sequential injections of hPAP (250 mU/injection) into popliteal fossa. (C, D) hPAP dose response. Thermal sensitivity measured in (C) hours and (D) days post hPAP injection (single injection of the indicated doses). (A-D) Ipsi = ipsilateral hindpaw. Contra = contralateral hindpaw. Contralateral popliteal fossa was not injected. Wild-type (n = 10) male mice in each cohort. Data are plotted as means ± s.e.m. Paired t-tests were used to compare responses at each time point to BL. *P < 0.05, **P < 0.005, ***P < 0.0005.