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Figure 2 | Molecular Pain

Figure 2

From: Mitogen activated protein kinase phosphatase-1 prevents the development of tactile sensitivity in a rodent model of neuropathic pain

Figure 2

In vitro characterization of rat MKP-1 overexpression. MKP-1 mRNA levels (a, qRT-PCR) and Western blot of MKP-1 protein (b) in normal BV-2 cells and stably MKP-1 overexpressing BV-2 cells. (c) Representative Western blots of p-p38, and p-JNK expression in BV-2 cells stably overexpressing MKP-1 following 1 or 2 h incubation in medium (control alone) or lipopolysaccharide (LPS) stimulation (1 μg/ml). IL-6 (d), TNF-α (e), nitrite (f), and MCP-1) (g) release in control (normal) and stably expressing MKP-1 BV-2 cells challenged with LPS (1 μg/ml) for 6 or 12 h. Results are expressed as mean ± s.e.m. of three experiments in triplicate. *P < 0.05 vs. control BV-2 cells.

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Molecular Pain

ISSN: 1744-8069

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