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Figure 1 | Molecular Pain

Figure 1

From: Lamina I NK1 expressing projection neurones are functional in early postnatal rats and contribute to the setting up of adult mechanical sensory thresholds

Figure 1

NK1-positive lamina I neurons express c-fos in response to injection of formalin in the hindpaw and project to the lateral parabrachial nucleus (PBN) in postnatal day 3 (P3) rats. A, Immunohistochemistry for NK1 (green) and c-fos (red) in the dorsal horn of a formalin-stimulated P3 rat. Colocalization of NK1 with c-fos can be seen. Scale bar, 40 μm. B, Fluorogold (FG) injection site in the lateral PBN. Arrows indicate the edge of the brain section. PB: parabrachial area. C, Retrograde labeling of lamina I-parabrachial neurons with FG in a P3 lumbar cord section at 24 hours post-injection. B and C: Scale bar, 200 μm. D, Immunohistochemistry for NK1 (green) and FG (red) in lamina I at 24 hours after injection of FG in the lateral PBN at P3. Co-localization of NK1 with FG is seen in yellow. Scale bar, 20 μm. E, Immunohistochemistry for c-fos (green) and FG (red) in lamina I of a formalin-stimulated P3 rat 1 day after parabrachial injection with FG. Co-localization of c-fos with FG can be seen in yellow. Scale bar, 40 μm. F, Number of lamina I neurons labeled with FG in P3 (N = 4) and adult rats (N = 3) injected with FG in the lateral PBN. G, Percentage of FG-labeled neurons in lamina I that co-localized with c-fos after injection of formalin in P3 (N = 4) and adult rats (N = 3). Data show group mean ± SEM. * p < 0.05.

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Molecular Pain

ISSN: 1744-8069

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