Intraplantar carrageenan results in significant disrupted spinal occludin in dorsal horn. Intraplantar carrageenan results in the appearance of disturbed occludin protein at 72 hours post-carrageenan in male (A) and female (B) rats. High magnification pictures show diffuse occludin staining and missing areas of overlap between occludin and VWF staining in both male (C) and female (D) rats. Blinded quantification reveals significant reduction of intact ipsilateral dorsal and contralateral dorsal occludin at 72 hrs post-carrageenan in male rats (E), but not in female rats (F). Pooled male and female groups show significant differences between vehicle and the 72 hour post-carrageenan treatment in dorsal horn (G). Subcutaneously administered carrageenan away from the hindpaw does not cause alterations in occludin protein. Abbreviations: subcutaneous (SC), others as in Figure 1 and 2.