Figure 3
From: Regulation of Wnt signaling by nociceptive input in animal models
Spatial distribution of Wnt3a in mouse SCDH. A. Immunostaining of Wnt3a in mouse spinal cord. Wnt3a (red) is detected throughout the entire gray matter, with more intensive label in the superficial laminae of SCDH. B. A merged image of Wnt3a and DAPI signals to show the distribution of Wnt3a-positive cells. C-D. Wnt3a staining (C) and double-staining of Wnt3a and SP (D). Wnt3a staining is observed in the SP-labeled (green) lamina I layer and the deeper regions in the SCDH. E-F. Wnt3a staining (E) and double-staining of Wnt3a and PKCγ (F). Wnt3a staining is detected in PKCγ-labeled (green) lamina II and probably lamina III. Scattered cells with strong Wnt3a staining are also in the deep SCDH laminae (arrows, C-F). Scale bars for A and B: 300 μm; Scale bars for C-F: 100 μm.