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Figure 4 | Molecular Pain

Figure 4

From: Activation of extracellular signal-regulated protein kinase 5 is essential for cystitis- and nerve growth factor-induced calcitonin gene-related peptide expression in sensory neurons

Figure 4

Retrograde NGF-induced CGRP expression was mediated by the MEK/ERK pathway but not by the PI3K/Akt pathway. In two-compartmented culture, the gangalia was pre-treated with vehicle (vehicle, A, B, E), or one of the inhibitors against the MEK/ERK pathway (C, F: U0126; G: PD98059), or the PI3K/Akt pathway (D, H: LY294002) followed by NGF treatment of the nerve terminals (B, C, D). After 12 h of NGF treatment, the level of CGRP was increased in the sensory neuronal cell bodies by 2 fold when compared to control (E). Both U0126 (F) and PD98059 (G: PD) pre-treatment blocked the retrograde NGF-facilitated CGRP expression in DRG. LY294002 (LY) pre-treatment did not affect the level of CGRP in the ganglia induced by retrograde NGF (H). Bar = 120 μm. *, p < 0.05 vs vehicle. Results were from 4 independent experiments for each treatment.

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Molecular Pain

ISSN: 1744-8069

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