Figure 6

Oxaliplatin pretreatment increases the number of AITC-sensitive DRG neurons. ( A- C) In cultured DRG neurons pretreated with vehicle or oxaliplatin at concentrations of 30 ( A), 100 ( B) or 300 μM ( C) for 1, 2, or 4 h, AITC (10 μM) was added for 3 min. ( D, E) In cultured DRG neurons pretreated with vehicle or oxaliplatin (100 μM) for 1, 2, or 4 h, menthol (100 μM; D) or capsaicin (500 nM; E) was added for 3 min, and the Ca²⁺ responses of the neurons were determined. Cells were considered responsive if their F₃₄₀/F₃₈₀ ratio increased by more than 0.2 during the 3-min application. The values show the percentage of agonist-sensitive cells in 50 mM KCl-positive neurons. Data are presented as the mean ± S.E.M. of 4–6 separate experiments. *p < 0.05, compared with corresponding vehicle-pretreatment.