Histograms showing the mechanical threshold (± SEM) in the lateral, center and medial territories of the affected hind paw glabrous skin as determined with the Von Frey test. The SNI group showed a significantly decreased mechanical withdrawal threshold in the medial and lateral foot sole at 2, 5 and 10 weeks PO compared to the Sham group. Center area of the foot sole of the SNI group showed a somewhat higher mechanical withdrawal threshold, which decreased in week 10 compared to the Sham group, which were not significant. N = 6 per group. One-way ANOVA with post-hoc Turkey test, unpaired t-test *: P < 0.05, **: P < 0.01, ***:P < 0.001.