Figure 1

Effect of HODE Antibodies on CFA-induced Thermal Allodynia. A 50uL solution of 1:1 CFA:saline was injected into the right hind paw of rats and 24 h later, heat hyperalgesia was measured using the radiant heat test on the inflamed paws as well as in the contralateral (un-inflamed) paws. Following this, in one group of animals, 25ug of anti- 9-HODE and 25ug of anti-13-HODE antibodies were injected into the inflamed paw. Heat hyperalgesia was measured at 15, 30 and 60 minutes after antibody injection in the A. inflamed paw and B. in the contralateral paw. Similarly, another group of animals was injected with same amount of denatured HODE antibodies. Heat hyperalgesia was measured at 15, 30 and 60 minutes after antibody injection in the C inflamed paw and D. in the contralateral paw. n = 6 was used for each group in all the experiments and mean of all n’s is plotted. Data were analyzed using one-way ANOVA with Neuman-Keuls Post-hoc test. Error Bar: S.E.M.