Figure 2

Effect of Ketoconazole on CFA-induced Thermal Allodynia. A 50uL solution of 1:1 CFA:saline was injected into the right hind paw of rats and 24 hours later, heat hyperalgesia was measured using the radiant heat test in the inflamed paws as well as in the contralateral paws. As noted, ketoconazole (4ug) was injected 30 sec prior to CFA injection and 24 hours later, thermal allodynia was measured in the A. inflamed paw and in the contralateral paw, following which another injection of ketoconazole (4ug) was injected in the inflamed paw. Heat hyperalgesia was tested at 30 and 60 minutes after second injection in the A inflamed paw and in the contralateral paw. In a separate group of animals, vehicle was injection 30 seconds prior to CFA injection. Heat hyperalgesia tested at 24 hours in the B. inflamed as well as contralateral paws, following which another vehicle injection was given and heat hyperalgesia tested at 30 and 60 minutes in the B. inflamed as well as in the contralateral paws. For data set C and D, the experiment was performed in the similar fashion as 2A and B, except C. ketoconazole or D. vehicle was injected in the un-inflamed paw and heat hyperalgesia measured in ipsilateral paw (inflamed) as well as contralateral paw, 30 and 60 minutes after second injection. n = 6 was used for each group in all the experiments and mean of all n’s is plotted. Data were analyzed using one-way ANOVA with Neuman-Keuls Post-hoc test. Error Bar: S.E.M.