Effect of 15d-PGJ
on mechanical- and heat-sensitivity. (A) 15 mM 15d-PGJ2 (ipl.) attenuates CFA-induced mechanical hypersensitivity. Von Frey measurements were recorded before CFA injection (Naïve), 24 h post-CFA injection and at noted time points. 15d-PGJ2 was administered 1 h prior to von Frey measurements. (B) 15d-PGJ2 shows no analgesic effect (no change latency to licking or flicking) against inflammatory heat hypersensitivity in Hargreaves test. (C) 15d-PGJ2 itself does not cause mechanical hypersensitivity (1 h after ipl. injection), while AITC does (*p < 0.05, **p < 0.01, *** p < 0.001; n = 8 per group; values expressed as mean ± SEM). Data were analyzed using RMANOVA with Bonferonni post-hoc comparisons (A) or two-tailed Student’s t-test (B and C).