Figure 2

SPS produced anxiety-like behavior in EPM test. Two sets of rats were tested for 5 min duration on EPM at day 9 (control: n=12, SPS: n=11) or 14 (control: n=8, SPS: n=7) after SPS. At day 9 SPS-exposed rats spent significantly less time in open arms (A. **p<0.01) and made fewer entries into open arms (B. *p<0.05), consistent with anxiety-like behavior. No difference between groups was noted at day 14 after SPS. No significant difference in total immobile time (C) or distance travelled (D) between control and SPS rats was observed at either time point. Statistical analysis was carried out by unpaired Student’s t-test. Data are presented as mean ± SEM.