Scgn -LI in control rat spinal dorsal horn. (a-f) Immunofluorescence micrographs after double-staining with antiserum against Scgn (a,c,d,f), CGRP (b,c) or IB4 (e,f). (a-f) Scgn-IR neurons (arrows) are seen in the dorsal horn, both in superficial and deeper layers (a,d). They partly overlap with CGRP-LI (b) and IB4 staining (e), as seen in merged color micrographs (c,f). (g-o) Double-immunofluorescence micrographs of sections incubated with Scgn antiserum (g,i,j,l,m,o; green) plus CB (h, i; red), PV (k,l; red) or CR antiserum (n,o; red). Coexistence is often seen for CB in cell bodies (arrows in g-i) and processes (arrowheads in g-i), less so for PV (arrow/arrowheads in j-l) and, here, none for CR (m-o). Arrows indicate coexistence (yellow) of Scgn-LI (i, l) and CB (i) and PV (l) in the dorsal horn neurons. Arrowheads indicate coexistence of Scgn-IR fibers (i,l) and CB (i) or PV (l). Curved arrows indicate a Scgn-positive neuron that does not express CB (g-i). Arrows indicate a CR-positive but Scgn negative neuron (m-o). Bar in f indicates 100 μm (a-f) and 50 μm (g-o).