Figure 3

Effects of 1,8-cineole on fura-2 ratio in HEK293T cells. (A-C) Fura-2 ratio changes upon 1,8-cineole (5 mM) application in cells expressing hTRPV1 (n = 65) (A), hTRPV2 (n = 26) (B) or hTRPV3 (n = 79) (C). CAP (capsaicin), LPC (lysophosphatidylcholine), 2-APB (2-aminoethoxydiphenyl borate). Horizontal bars indicate duration of the applied stimuli. (D) 1,8-cineole caused significant fura-2 ratio increases in HEK293T cells expressing hTRPM8 (n = 32) or hTRPV3 (n = 79), but not in cells expressing hTRPV1 (n = 65), hTRPV2 (n = 26) or hTRPA1 (n = 41). Statistical significance was evaluated using ANOVA followed by two-tailed multiple t-test with Bonferroni correction. *: p < 0.05.