Figure 4
From: Effect of cholesterol depletion on the pore dilation of TRPV1
No effect of cholesterol depletion for 90 min treatment with 2.5 mM M β CD:cholesterol (10:1) on heat-activated TRPV1 currents. (A) The uptake rate of YO-PRO mediated by TRPV1 during sustained activation with heat (45°C) of cells in extracellular buffer 8, was unaffected by treatment with MβCD:cholesterol (n = 80–94). (B) Representative trace of a TRPV1 whole-cell response (bottom) from a cell clamped at −60mV (extracellular buffer 6, intracellular buffer 7), elicited by a heat-protocol (top) where temperature was ramped from room temperature (∼20°C) to 38°C, then stepped upwards by 1°C to 49°C, held at 10 s per step. (C) Heat-activated TRPV1 current densities were unaffected by treatment with MβCD:cholesterol (n = 5–9).