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Figure 6 | Molecular Pain

Figure 6

From: Intervention of electroacupuncture on spinal p38 MAPK/ATF-2/VR-1 pathway in treating inflammatory pain induced by CFA in rats

Figure 6

Effect of EA on COX-2 expression in the ipsilateral spinal dorsal horn at 14 day after CFA injection. A. Expression of COX-2 mRNA (relative to GAPDH) in L4-6 ipsilateral spinal cord dorsal horn measured by qPCR. Standard curve of COX-2 and GAPDH mRNA in the ipsilateral spinal cord dorsal horn is shown in Additional file 1: Figure S3. It shows that relative quantity of COX-2 mRNA has no significant difference among Control group, CFA group, CFA+EA group and CFA+Sham EA group on day 14 after CFA injection. n=5-7. B, C. Western blotting analysis reveals that COX-2 protein in the L4-6 ipsilateral spinal dorsal horn has also no significant difference among Control group, CFA group, CFA+EA group and CFA+Sham EA group on 14 day after CFA injection. C. Quantification of B. COX-2 protein in normalized against β-actin. n=4-6.

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