Figure 2
Voltage gated Ca2+current of DiI+DRG neurons from control and TNBS treated rats. A, The photomicrograph of dissociated L6-S2 DRG neurons. B, DiI-labeled neuron (arrow) is visualized under epifluorescence illumination. C, Representative total I Ba currents traces of DiI+ neurons from control rats. D, Representative total I Ba currents traces of DiI+ neurons from TNBS treated rats. E, Current–voltage relationships (I-V curves). Current–voltage plot of average data shows an increase of peak current in colonic neurons from TNBS treated rats (open circle) compared with control rats (square). F, Bar graph shows peak current density of I Ba currents. The average peak I Ba currents of colonic neurons from TNBS treated rats were significantly increased compared with control rats (−127.82 ± 20.82 pA/pF Vs −91.67 ± 19.02 pA/pF, n = 9 for both; * P < 0.05). Scale bar for A and B =30 μ m.