Figure 3

Detection of NCX isoforms in rat TG cells by real-time RT-PCR. PCR products were monitored by the increase in fluorescence caused by binding of SYBR green dye to double-stranded DNA. Data was analyzed by the 2^{−[ΔΔ Ct]}method, with GAPDH as an internal control (which was positive in all samples). Note that the relative mRNA expression level of NCX isoforms does not indicate the absolute level of expression. Each bar denotes mean ± S.D. of mRNA expression relative to GAPDH. Statistically significant differences between columns are indicated by asterisk: *p<0.05.