Figure 4

Strength of immunostaining for nNOS and galanin among sst _2A ⁺and GFP⁺neurons in laminae I-II of the PrP-GFP mouse. a, The frequency of cells with different intensities of labelling for nNOS and galanin, graded from 4 (strong) to 1 (very weak), or 0 (negative). Data were pooled from 4 mice (two dorsal horns from each mouse). Although many cells were only immunoreactive for nNOS or galanin, some showed both types of immunoreactivity. b, The equivalent frequency histogram for all of the cells shown in a that were also GFP-labelled.