Figure 5

GFP intensity among galanin and nNOS cells in the superficial dorsal horn of the PrP-GFP mouse and their laminar distribution. The GFP⁺ cells that contained either galanin or nNOS were divided into 3 groups: those that had a higher intensity score for galanin (galanin), those with a higher score for nNOS (nNOS) and those for which the scores were equal (both). a The numbers of cells belonging to each group that were defined as having weak (1), medium (2), strong (3) or very strong (4) labelling for GFP. Each column shows the mean number of cells per mouse and the range across the 4 mice. Note that cells in the galanin group tended to have a low level of GFP, while those in the nNOS group often had high GFP levels. b The laminar location of cells in these 3 groups plotted onto an outline of the superficial dorsal horn. In each drawing the upper line represents the edge of the dorsal horn and the lower line the lamina II-III border. Cells belonging to the nNOS group are shown as open circles, those in the galanin group as filled circles and those defined as both as filled triangles. The lower right drawing shows all 3 groups combined.