Figure 5

CCL2 protein expression at day 14 in the MIA model. The amount of CCL2 protein was measured in vehicle and MIA animals 14 days after treatment in the cartilage (A), subchondral bone (B) and fat pad (C). A large increase in CCL2 protein was measured in the cartilage (A) although when compared to vehicle treated animals this was not statistically significant. Significant increases in CCL2 protein was measured in the subchondral bone (B) and fat pad (C). The protein concentration shown is normalised to 1 mg/ml of total protein. T-test, **p < 0.01, n = 3 (A), n = 6 (B and C). All data are expressed as mean ± SEM.