Figure 4

Intracelluar pathway downstream to D1/D5 dopamine receptors activation. A) Collected results showing the effect of 10 μM SKF 81297 on TTX-R sodium current applied either alone (circles) or in combination with 10 μM PKAi(6–22) (squares) included in the patch pipette to block the PKA activity. B) The inhibitory effect of 10 μM SKF 81297 on the relative peak of TTX-R sodium current was slightly reduced from 0.63 ± 0.08 (n = 22) when applied alone to 0.66 ± 0.06 (n = 11, t-test, two populations, p = 0.229) when applied in combination with 10 μM PKAi(6–22) included in the patch pipette. C) Collected results showing the effect of 10 μM SKF 81297 on TTX-R sodium current applied either alone (circles) or in combination with 5 μM PKCi(19–36) (triangles) included in the patch pipette to block the PKC activity. D) The inhibitory effect of 10 μM SKF 81297 on the relative peak of TTX-R sodium current was significantly reduced from 0.63 ± 0.08 (n = 22) when applied alone to 0.78 ± 0.06 (n = 11, t-test, two populations, **p < 0.01) when applied in combination with 5 μM of PKCi(19–36) included in the patch pipette.