Figure 2

Aδ-Mechanoreceptor and C fibers in SNI mice exhibit enhanced mechanical firing. Using ex vivo skin nerve recordings, 10 sec mechanical stimuli ranging 5-200 mN were applied to the receptive field of each fiber using a 0.8 mm probe. All recordings were performed on the sural nerve and its innervating territory. A) Aδ-Mechanoreceptor (AM) fibers fired an average 22% more action potentials per second across all forces compared to sham or to naïve animals (**p<0.01). B) Examples of AM fiber action potentials evoked by sustained mechanical stimuli in naïve, sham and SNI treatment groups. C) C fibers fired an average 24% more action potentials per second across all forces compared to sham and 28% more compared to naïve animals (*p<0.05). D) Examples of C fiber action potentials evoked by sustained mechanical stimuli in naïve, sham and SNI treatment groups. Number of action potentials fired per second was compared across all forces and treatment groups using a 2-way ANOVA with Bonferroni post hoc comparisons. Error bars indicate S.E.M.